PUMA 3D Printed Microscope

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PJT
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Joined: Sun Aug 15, 2021 9:39 pm

Re: PUMA 3D Printed Microscope

Post by PJT »

The latest PUMA Microscope video is out (see https://youtu.be/1XmScHUicOs ) which shows you how to build the trans-polarising illumination system. Below is the FreeCAD design (together and exploded) with the real world 3D printed version bottom left and an example of polarised microscopy of citric acid crystals taken with the microscope using this system. The project github page is: https://github.com/TadPath/PUMA
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User avatar
PJT
Posts: 11
Joined: Sun Aug 15, 2021 9:39 pm

Re: PUMA 3D Printed Microscope

Post by PJT »

The latest PUMA microscope video ( https://youtu.be/XEE-el7vC5k ) is out and discusses Köhler illumination - the method of illumination used by most professional light microscopes today. After a historical introduction going over Dr Köhler's original paper (written in German - English translation also provided) I show you some of Köhler's original photomicrographs from the day (1893) and then go on to show how to build your own Köhler illuminator from scratch for the PUMA microscope (all designed in FreeCAD of course - although I used a different package to do the optical path CAD).
I know this will be a bit too specialist unless you are a microscopist but I think there are lessons to be learnt re designing practical instruments with FreeCAD and 3D printing hence I post about it here.
All specs and FreeCAD files are freely available on the open source PUMA microscope GitHub page: https://github.com/TadPath/PUMA
Enjoy.
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